GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318253/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318253GEOGSEfalseTET1 non-catalytic activity shapes the chromatin-landscape that directs de novo methylation establishment in the male germline [CUTandRUN]In the germline, DNA methylation is globally erased in primordial germ cells (PGCs), enabling establishment of sex-specific methylomes in prospermatogonia or oocytes. The catalytic activity of TET1 is required for complete demethylation in PGCs, yet sperm from Tet1-/- mice display methylation defects not explained by incomplete erasure. Instead, these defects arise from abnormal de novo methylation during development, coinciding with erosion of H3K4me3, a chromatin modification that blocks DNMT3A/3L. Using a catalytically inactive Tet1HxD mouse line, we demonstrate a non-catalytic role of TET1 in promoting H3K4me3 deposition and protecting a subset of sperm hypomethylated regions from aberrant methylation in prospermatogonia.2026/07/22GSE318253GSM9491413GSM9491402GSM9491403GSM9491414GSM9491411GSM9491400GSM9491401GSM9491412GSM9491406GSM9491407GSM9491404GSM9491415GSM9491405GSM9491416GSM9491408GSM9491409GSM9491399GSM949141032159318253Mus musculus[42448565]