{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318304/suppl/GSE318304_RawCount_Matrix_ann.txt.gz"],"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318304/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318304"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RXR agonist increases newly characterized GM-CSF-producing B220+CD8αα interepithelial T cells","description":"Context-dependent cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a crucial role in maintaining intestinal immune homeostasis and exacerbating inflammation. Although multiple cell types produce GM-CSF in the gut, whether intraepithelial lymphocytes (IELs) contribute to GM-CSF production during the steady state remains unclear. In the small intestinal epithelial layer, we identified B220-expressing CD8αα T cells that selectively produced GM-CSF but not interleukin-17A or interferon-gamma. Transcriptional analysis combined with weighted parametric gene set analysis revealed the upregulation of retinoid X receptor (RXR)-related genes in this population. Consistently, the RXR agonist bexarotene increased GM-CSF-producing B220+CD8αα T cells without affecting other cytokines or CD8αβ T cells. These findings identify B220+CD8αα IELs as a spatially distinct source of GM-CSF in the epithelial layer and indicate that the RXR pathway specifically facilitates GM-CSF-producing program, potentially contributing to maintaining immune homeostasis in the small intestine.","dates":{"publication":"2026/06/19"},"accession":"GSE318304","cross_references":{"GSM":["GSM9492029","GSM9492032","GSM9492033","GSM9492030","GSM9492031","GSM9492034"],"GPL":["24247"],"GSE":["318304"],"taxon":["Mus musculus"]}}