{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318306/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Non-coding RNA profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318306"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Whole transcriptome sequencing of HCT116 cells with altered DNA repair capacities treated with RTX or 5FdUR","description":"Thymidylate synthase (TS) is a key enzyme in thymidylate biosynthesis and an established target of chemotherapeutics such as 5-fluoro-2’-deoxyuridine (5FdUR) and raltitrexed (RTX). Inhibition of TS disrupts the dUTP:dTTP balance, leading to uracil misincorporation, futile base excision repair cycles, DNA strand breaks, and ultimately cell death. However, active U-DNA repair is apparently not required for the cytotoxic effect of these drugs, indicating that alternative pathways contribute to the observed S-phase arrest. To dissect these mechanisms, we investigated the transcriptomic effects of TS inhibition in HCT116 colon cancer cell lines with altered DNA repair capacities treated with 5-fluoro-2′-deoxyuridine (5FdUR) or raltitrexed (RTX). Both drugs induced similar DNA damage responses and S-phase arrest, yet displayed distinct transcriptional signatures. Our findings underscore the multifaceted impact of TS inhibition and reveal drug-specific differences in cellular responses.","dates":{"publication":"2026/07/08"},"accession":"GSE318306","cross_references":{"GSM":["GSM9492049","GSM9492050","GSM9492072","GSM9492051","GSM9492073","GSM9492070","GSM9492071","GSM9492054","GSM9492076","GSM9492055","GSM9492052","GSM9492074","GSM9492075","GSM9492053","GSM9492058","GSM9492059","GSM9492056","GSM9492057","GSM9492061","GSM9492062","GSM9492060","GSM9492043","GSM9492065","GSM9492044","GSM9492066","GSM9492041","GSM9492063","GSM9492064","GSM9492042","GSM9492047","GSM9492069","GSM9492048","GSM9492067","GSM9492045","GSM9492046","GSM9492068"],"GPL":["24676"],"GSE":["318306"],"taxon":["Homo sapiens"],"PMID":["[42384741]"]}}