GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318410/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318410GEOGSEfalseThe effects of RBM27 on heart functionThe goal of this study is to investigate how RBM27 regulates heart function. Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from hearts of Rbm27flox/flox and Rbm27-CKO mice at 12 weeks old. Three independent biological replicates for each group were used for RNA-seq. RNA-seq was performed by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm38/mm10) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3.2026/08/18GSE318410GSM9494024GSM9494023GSM9494022GSM9494027GSM9494026GSM949402524247318410Mus musculus