{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318642/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318642"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Electrically Preconditioned Neural Tissues Direct Circuit Reconstruction for Spinal Cord Repair","description":"Spinal cord injury (SCI) creates a prohibitive microenvironment that limits the efficacy of neural stem cell (NSC) therapies. We developed electrically preconditioned engineered neural tissues (ENT) to address these limitations through: (1) pre-establishment of functional neural networks in vitro, and (2) enhanced host integration capacity. EGFP-expressing NSCs were differentiated in 3D Matrigel under 150 mV/mm physiological electric fields (EFs) and transplanted into T10 hemisection SCI mice. Outcomes were assessed through: Basso Mouse Scale (BMS) scoring, multiplex immunofluorescence (Nestin/MAP2/GFAP/MBP/Synaptophysin/ChAT), cortical somatosensory/motor evoked potentials (CSEP/CMEP), RNA sequencing and pathway analysis. At 28 days post transplantation, ENT transplanted animals showed significantly higher BMS scores and enhanced CSEP/CMEP amplitudes compared to the NSC suspension group. We conducted comprehensive evaluations of the histological structure and function of EF-preconditioned ENT and the mice that received ENT transplantation: (1) in vitro maturation of ENT: high neuronal differentiation, dense synaptic networks and myelinated axon; (2) in vivo integration: niche-directed migration (graft-derived cells showed central canal (Nestin+ cells) and grey matter (ChAT+ cells) homing), achieved functional synaptic integration and correlated with motor recovery. Mechanistic analysis revealed EF activation of pro-neuronal pathways and gliogenesis suppression. These results demonstrate that EF-preconditioned ENT contributes to structural neural network reconstruction, niche-directed homing, functional synaptic integration and significant motor recovery.","dates":{"publication":"2026/04/01"},"accession":"GSE318642","cross_references":{"GSM":["GSM9499701","GSM9499699","GSM9499700","GSM9499698"],"GPL":["24247"],"GSE":["318642"],"taxon":["Mus musculus"]}}