GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318642/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318642GEOGSEfalseElectrically Preconditioned Neural Tissues Direct Circuit Reconstruction for Spinal Cord RepairSpinal cord injury (SCI) creates a prohibitive microenvironment that limits the efficacy of neural stem cell (NSC) therapies. We developed electrically preconditioned engineered neural tissues (ENT) to address these limitations through: (1) pre-establishment of functional neural networks in vitro, and (2) enhanced host integration capacity. EGFP-expressing NSCs were differentiated in 3D Matrigel under 150 mV/mm physiological electric fields (EFs) and transplanted into T10 hemisection SCI mice. Outcomes were assessed through: Basso Mouse Scale (BMS) scoring, multiplex immunofluorescence (Nestin/MAP2/GFAP/MBP/Synaptophysin/ChAT), cortical somatosensory/motor evoked potentials (CSEP/CMEP), RNA sequencing and pathway analysis. At 28 days post transplantation, ENT transplanted animals showed significantly higher BMS scores and enhanced CSEP/CMEP amplitudes compared to the NSC suspension group. We conducted comprehensive evaluations of the histological structure and function of EF-preconditioned ENT and the mice that received ENT transplantation: (1) in vitro maturation of ENT: high neuronal differentiation, dense synaptic networks and myelinated axon; (2) in vivo integration: niche-directed migration (graft-derived cells showed central canal (Nestin+ cells) and grey matter (ChAT+ cells) homing), achieved functional synaptic integration and correlated with motor recovery. Mechanistic analysis revealed EF activation of pro-neuronal pathways and gliogenesis suppression. These results demonstrate that EF-preconditioned ENT contributes to structural neural network reconstruction, niche-directed homing, functional synaptic integration and significant motor recovery.2026/04/01GSE318642GSM9499701GSM9499699GSM9499700GSM949969824247318642Mus musculus