{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318768/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Methylation profiling"],"species":["Homo sapiens"],"gds_type":["Methylation profiling by genome tiling array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318768"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"HPV-related DNA methylation alterations shape head and neck tumor microenvironment in space and time","description":"This study explores DNA methylation profile and their impact on tumor microenvironment (TME) of head and neck cancer (HNC). By integrating advanced high-throughput platforms, including Human Methylation 450K BeadChip microarrays and single-cell imaging mass cytometry (IMC), we identified distinct methylation signatures and immune landscapes in HNC associated with HPV-positive vs. HPV-negative tumors. Differentially methylated sites between HPV-positive and HPV-negative tumors showed enrichment for immune system regulatory pathways, with marked signatures of T lymphocyte regulation. To better understand the relationship between DNA methylation and tumor microenvironment regulation, we sought to evaluate the immunological components from a spatial perspective and observed that in HPV-positive tumors, T lymphocytes appear to activate a self-regulatory mechanism, while in HPV-negative tumors this mechanism is blocked by the action of fibroblasts, which are the cells that interact most with T lymphocytes. The spatial dynamics within the TME regulated by epigenetic mechanisms pave the way for more precise clinical decision-making and personalized therapies for HNC patients.","dates":{"publication":"2026/02/11"},"accession":"GSE318768","cross_references":{"GSM":["GSM9502854","GSM9502843","GSM9502844","GSM9502855","GSM9502845","GSM9502856","GSM9502857","GSM9502846","GSM9502847","GSM9502858","GSM9502859","GSM9502848","GSM9502849","GSM9502860","GSM9502850","GSM9502861","GSM9502851","GSM9502841","GSM9502852","GSM9502842","GSM9502853"],"GPL":["13534"],"GSE":["318768"],"taxon":["Homo sapiens"]}}