GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318768/primaryOK200Methylation profilingHomo sapiensMethylation profiling by genome tiling arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318768GEOGSEfalseHPV-related DNA methylation alterations shape head and neck tumor microenvironment in space and timeThis study explores DNA methylation profile and their impact on tumor microenvironment (TME) of head and neck cancer (HNC). By integrating advanced high-throughput platforms, including Human Methylation 450K BeadChip microarrays and single-cell imaging mass cytometry (IMC), we identified distinct methylation signatures and immune landscapes in HNC associated with HPV-positive vs. HPV-negative tumors. Differentially methylated sites between HPV-positive and HPV-negative tumors showed enrichment for immune system regulatory pathways, with marked signatures of T lymphocyte regulation. To better understand the relationship between DNA methylation and tumor microenvironment regulation, we sought to evaluate the immunological components from a spatial perspective and observed that in HPV-positive tumors, T lymphocytes appear to activate a self-regulatory mechanism, while in HPV-negative tumors this mechanism is blocked by the action of fibroblasts, which are the cells that interact most with T lymphocytes. The spatial dynamics within the TME regulated by epigenetic mechanisms pave the way for more precise clinical decision-making and personalized therapies for HNC patients.2026/02/11GSE318768GSM9502854GSM9502843GSM9502844GSM9502855GSM9502845GSM9502856GSM9502857GSM9502846GSM9502847GSM9502858GSM9502859GSM9502848GSM9502849GSM9502860GSM9502850GSM9502861GSM9502851GSM9502841GSM9502852GSM9502842GSM950285313534318768Homo sapiens