{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318861/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318861"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative analysis of Tat-dependent and host-driven HIV transcription by ChIP-seq and RNA-seq","description":"HIV transcription is initiated by host transcriptional machinery prior to the production of the viral transactivator Tat, yet the magnitude and regulatory features of this Tat-independent transcriptional state remain poorly defined. In this study, we performed integrated chromatin and transcriptional profiling to quantitatively compare Tat-dependent and host-driven regulation of HIV and cellular gene expression. Jurkat T cells were infected with isogenic HIV constructs expressing functional Tat (TatWT) or lacking Tat expression (TatNull) and analyzed under non-stimulated and stimulated conditions. Genome-wide chromatin occupancy of Tat and transcriptional machinery was measured by ChIP-seq, and corresponding transcriptional output from both the HIV provirus and host genome was quantified by RNA sequencing. These datasets define the baseline host-driven transcriptional state of HIV in the absence of Tat and enable direct comparison with Tat-amplified transcriptional responses. Together, this integrated ChIP-seq and RNA-seq resource provides a quantitative framework for dissecting Tat-dependent and Tat-independent mechanisms of HIV transcriptional regulation in chromatin.","dates":{"publication":"2026/07/01"},"accession":"GSE318861","cross_references":{"GSM":["GSM9504893","GSM9504892","GSM9504895","GSM9504894","GSM9504930","GSM9504897","GSM9504896","GSM9504899","GSM9504898","GSM9504891","GSM9504890","GSM9504923","GSM9504889","GSM9504922","GSM9504925","GSM9504924","GSM9504927","GSM9504926","GSM9504929","GSM9504928","GSM9504882","GSM9504881","GSM9504884","GSM9504883","GSM9504886","GSM9504885","GSM9504921","GSM9504888","GSM9504887","GSM9504920","GSM9504880","GSM9504919","GSM9504912","GSM9504879","GSM9504911","GSM9504914","GSM9504913","GSM9504916","GSM9504915","GSM9504918","GSM9504917","GSM9504910","GSM9504909","GSM9504908","GSM9504901","GSM9504900","GSM9504903","GSM9504902","GSM9504905","GSM9504904","GSM9504907","GSM9504906"],"GPL":["30173"],"GSE":["318861"],"taxon":["Homo sapiens"],"PMID":["[42328785]"]}}