GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318861/primary200OKGenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318861GEOGSEfalseQuantitative analysis of Tat-dependent and host-driven HIV transcription by ChIP-seq and RNA-seqHIV transcription is initiated by host transcriptional machinery prior to the production of the viral transactivator Tat, yet the magnitude and regulatory features of this Tat-independent transcriptional state remain poorly defined. In this study, we performed integrated chromatin and transcriptional profiling to quantitatively compare Tat-dependent and host-driven regulation of HIV and cellular gene expression. Jurkat T cells were infected with isogenic HIV constructs expressing functional Tat (TatWT) or lacking Tat expression (TatNull) and analyzed under non-stimulated and stimulated conditions. Genome-wide chromatin occupancy of Tat and transcriptional machinery was measured by ChIP-seq, and corresponding transcriptional output from both the HIV provirus and host genome was quantified by RNA sequencing. These datasets define the baseline host-driven transcriptional state of HIV in the absence of Tat and enable direct comparison with Tat-amplified transcriptional responses. Together, this integrated ChIP-seq and RNA-seq resource provides a quantitative framework for dissecting Tat-dependent and Tat-independent mechanisms of HIV transcriptional regulation in chromatin.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 sapiens[42328785]