GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319129/primaryOK200Methylation profilingMus musculusMethylation profiling by genome tiling arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319129GEOGSEfalseTET1 non-catalytic activity shapes the chromatin landscape that directs de novo methylation establishment in the male germline [methylation array]In the germline, DNA methylation is globally erased in primordial germ cells (PGCs), enabling establishment of sex-specific methylomes in prospermatogonia or oocytes. The catalytic activity of TET1 is required for complete demethylation in PGCs, yet sperm from Tet1-/- mice display methylation defects not explained by incomplete erasure. Instead, these defects arise from abnormal de novo methylation during development, coinciding with erosion of H3K4me3, a chromatin modification that blocks DNMT3A/3L. Using a catalytically inactive Tet1HxD mouse line, we demonstrate a non-catalytic role of TET1 in promoting H3K4me3 deposition and protecting a subset of sperm hypomethylated regions from aberrant methylation in prospermatogonia.2026/07/22GSE319129GSM9511531GSM9511532GSM9511540GSM9511530GSM9511539GSM9511528GSM9511529GSM9511526GSM9511537GSM9511527GSM9511524GSM9511535GSM9511536GSM9511533GSM9511523GSM951153431191319129Mus musculus[42448565]