{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319380/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319380"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic and phenotypic data from patient-derived astrocytes in sporadic Alzheimer’s disease","description":"Astrocytes are essential regulators of central nervous system homeostasis and actively participate in the progression of neurodegenerative diseases, including Alzheimer’s disease (AD). While familial AD (fAD) has been linked to defined genetic mutations, sporadic AD (sAD)—the predominant and more heterogeneous form—lacks clear causative mechanisms. Human-induced pluripotent stem cells (hiPSCs) offer a powerful platform for modelling sAD by enabling the generation of patient-specific neural cell types under controlled conditions. In this dataset, we present a detailed characterisation of hiPSC-derived astrocytes from individuals with clinically diagnosed sAD and age-matched healthy controls. Cells were exposed to either aggregated amyloid-beta (Aβ) or hydrogen peroxide (H₂O₂) to mimic disease-relevant stressors associated with proteotoxicity and oxidative damage. We report (1) genome-wide transcriptional responses assessed by bulk RNA sequencing, (2) IL-6 cytokine secretion as a marker of inflammatory activation, and (3) phenotypic outcomes, including morphological alterations and cell viability. This dataset provides a valuable resource for investigating astrocyte-specific contributions to sAD pathophysiology and offers insights into how patient-derived astrocytes respond to key neurodegenerative triggers. All data, protocols, and metadata are openly available to support reproducibility and further exploration by the neuroscience and stem cell research communities.","dates":{"publication":"2026/08/16"},"accession":"GSE319380","cross_references":{"GSM":["GSM9516923","GSM9516901","GSM9516902","GSM9516924","GSM9516925","GSM9516903","GSM9516904","GSM9516926","GSM9516905","GSM9516927","GSM9516928","GSM9516906","GSM9516907","GSM9516929","GSM9516908","GSM9516920","GSM9516921","GSM9516922","GSM9516909","GSM9516934","GSM9516912","GSM9516913","GSM9516935","GSM9516936","GSM9516914","GSM9516915","GSM9516916","GSM9516917","GSM9516918","GSM9516919","GSM9516930","GSM9516931","GSM9516910","GSM9516932","GSM9516911","GSM9516933"],"GPL":["24676"],"GSE":["319380"],"taxon":["Homo sapiens"]}}