GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319489/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319489GEOGSEfalseRestored clearance of senescent neutrophils by tissue-resident macrophages limits organ agingSingle-cell RNA sequencing of sorted mouse liver cells from young (6-8 month) and aged (23-25 month) Cx3cr1CreER;EP2wt/wt (WT) and Cx3cr1CreER;EP2lox/lox (EP2 cKO) male mice. Liver cells were enzymatically dissociated and live CD45+, CD1d+, or CD31+ cells were FACS-sorted in a 1:1:1 ratio, then processed using the 10x Genomics Chromium X platform (Next GEM Single Cell 3' Gene Expression). This dataset supports the finding that tissue-resident macrophage (TRM)-selective EP2 (PTGER2) deletion restores efferocytic clearance of senescent neutrophils and reverses age-associated transcriptional programs in Kupffer cells, preventing multi-organ aging phenotypes.2026/07/16GSE319489GSM9518591GSM9518590GSM9518593GSM9518592GSM9518586GSM9518588GSM9518587GSM951858934328319489Mus musculus