{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319524/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":[" Saccharomyces cerevisiae","Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319524"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcription-coupled repair removes cytotoxic DNA methylation damage and promotes mutational strand asymmetry in gliomas","description":"Transcription-coupled nucleotide excision repair (TC-NER) is known to repair bulky DNA adducts such as UV damage. However, whether TC-NER repairs small base damage is less understood. Alkylating agents induce cytotoxic base modifications (e.g., methylation) and are widely used in chemotherapy. Here we used a genome-wide methylation damage mapping method, N-methylpurine sequencing 2.0 (NMP-seq 2.0), and generated high-resolution methylation damage and repair profiles in human cells treated by methyl methanesulfonate (MMS). Our data revealed a repair strand asymmetry in base excision repair (BER) deficient cells lacking the N-methylpurine DNA glycosylase (MPG, also known as AAG), with higher repair rate on the transcribed strand (TS) relative to the non-transcribed strand (NTS). MMS induces N7-methylguanine (7meG) and N3-methyladenine (3meA). Interestingly, the repair asymmetry was only found for 3meA, but not for 7meG, which is less cytotoxic and mutagenic than 3meA. Further knockout of TC-NER genes, CSB or UVSSA, significantly sensitized AAG-deficient cells to MMS and abolished the repair asymmetry, indicating that the TS-biased repair of 3meA is dependent on TC-NER. The methylating agent temozolomide (TMZ) is used for glioma treatment. By analyzing glioma mutation data, we found that TMZ-treated gliomas accumulated fewer G>A mutations on the TS relative to the NTS, a phenomenon that can be attributed to strand-biased repair of O6-methylguanine (O6meG) by TC-NER. Finally, we show that knockout of the TC-NER factor CSB significantly sensitized glioma cells to TMZ. These findings indicate that TC-NER repairs cytotoxic methylation damage (e.g., 3meA and O6meG) on the TS, promotes mutational strand asymmetry in TMZ-treated gliomas, and maintains resistance to alkylating agents.","dates":{"publication":"2026/04/30"},"accession":"GSE319524","cross_references":{"GSM":["GSM9519076","GSM9519098","GSM9519077","GSM9519074","GSM9519096","GSM9519097","GSM9519075","GSM9519078","GSM9519079","GSM9519090","GSM9519091","GSM9519094","GSM9519073","GSM9519095","GSM9519092","GSM9519093","GSM9519087","GSM9519088","GSM9519085","GSM9519086","GSM9519089","GSM9519080","GSM9519083","GSM9519084","GSM9519081","GSM9519082"],"GPL":["24676","18249"],"GSE":["319524"],"taxon":[" Saccharomyces cerevisiae","Homo sapiens"]}}