GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319528/primaryOK200OtherEscherichia coli BW25113Otherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319528GEOGSEfalseA Course-Based Undergraduate Research Experience (CURE) Using Transposon-directed insertion sequencing (TraDIS) to Identify Genes Required for Bacteriophage P1 Propagation in Escherichia coliUnderstanding gene function through forward genetic screens remains foundational to microbial genetics. Here, we describe the design and implementation of a multi-week course-based undergraduate research experience (CURE) embedded within a third-year Microbial Genetics course. In this CURE, students construct a high-density Tn5 transposon insertion library in Escherichia coli, challenge the library with bacteriophage P1, and use Transposon-Directed Insertion-site Sequencing (TraDIS) coupled with statistical analysis (edgeR) to identify genes required for phage propagation. Students perform transposome electroporation, phage infection assays, genomic DNA extraction, high-throughput sequencing data processing in Galaxy, differential insertion analysis, visualization in Artemis, and Gene Ontology enrichment analysis. The project integrates wet-lab microbiology, molecular genetics, and bioinformatics while modeling an authentic forward genetic screen. This CURE emphasizes experimental design, genome-wide functional analysis, statistical reasoning, and interpretation of large-scale sequencing data. The curriculum is adaptable to other bacterial species, phage systems, or selective pressures and provides a scalable framework for integrating authentic genomics research into upper-level microbiology courses.2026/09/09GSE319528GSM9519242GSM9519245GSM9519243GSM951924418995319528Escherichia coli BW25113