{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319612/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319612"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Rothia mucilaginosa Alleviates Constipation through Gut Microbiota Remodeling and Neuroactive Pathway Activation","description":"Constipation affects approximately 15% of the global population, and gut microbiota dysbiosis is implicated in its pathogenesis. Rothia mucilaginosa, a commensal bacterium with established anti-inflammatory properties, has not been previously investigated for its effects on intestinal function. In this study, we evaluated the therapeutic potential of R. mucilaginosa in a loperamide-induced constipation mouse model using multiomics approaches. Twenty-six SPF male C57BL/6 mice were divided into normal control (NC, n=8), constipation model control (MC, n=8), and R. mucilaginosa-treated (RG, n=10) groups. R. mucilaginosa intervention significantly improved fecal output and induced gut microbiota remodeling, including enrichment of Akkermansia muciniphila and Alistipes finegoldii. To characterize host molecular responses, RNA-seq was performed on colon tissues to identify differentially expressed genes and pathways associated with constipation alleviation, with particular focus on neuroactive pathway activation.","dates":{"publication":"2026/04/27"},"accession":"GSE319612","cross_references":{"GSM":["GSM9521330","GSM9521352","GSM9521353","GSM9521331","GSM9521332","GSM9521354","GSM9521333","GSM9521355","GSM9521350","GSM9521351","GSM9521338","GSM9521339","GSM9521334","GSM9521335","GSM9521336","GSM9521337","GSM9521341","GSM9521342","GSM9521343","GSM9521344","GSM9521340","GSM9521349","GSM9521345","GSM9521346","GSM9521347","GSM9521348"],"GPL":["24247"],"GSE":["319612"],"taxon":["Mus musculus"]}}