GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319612/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319612GEOGSEfalseRothia mucilaginosa Alleviates Constipation through Gut Microbiota Remodeling and Neuroactive Pathway ActivationConstipation affects approximately 15% of the global population, and gut microbiota dysbiosis is implicated in its pathogenesis. Rothia mucilaginosa, a commensal bacterium with established anti-inflammatory properties, has not been previously investigated for its effects on intestinal function. In this study, we evaluated the therapeutic potential of R. mucilaginosa in a loperamide-induced constipation mouse model using multiomics approaches. Twenty-six SPF male C57BL/6 mice were divided into normal control (NC, n=8), constipation model control (MC, n=8), and R. mucilaginosa-treated (RG, n=10) groups. R. mucilaginosa intervention significantly improved fecal output and induced gut microbiota remodeling, including enrichment of Akkermansia muciniphila and Alistipes finegoldii. To characterize host molecular responses, RNA-seq was performed on colon tissues to identify differentially expressed genes and pathways associated with constipation alleviation, with particular focus on neuroactive pathway activation.2026/04/27GSE319612GSM9521330GSM9521352GSM9521353GSM9521331GSM9521332GSM9521354GSM9521333GSM9521355GSM9521350GSM9521351GSM9521338GSM9521339GSM9521334GSM9521335GSM9521336GSM9521337GSM9521341GSM9521342GSM9521343GSM9521344GSM9521340GSM9521349GSM9521345GSM9521346GSM9521347GSM952134824247319612Mus musculus