{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319706/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319706"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spatial polarization of endothelial ICAM-1 drives CD8⁺ T cell exclusion in melanoma [Xenium]","description":"An immunosuppressive tumor microenvironment (TME) constrains therapeutic efficacy and worsens prognosis. Beyond T-cell abundance and functionality, their spatial proximity to malignant cells is a critical determinant of tumor control. However, the vascular mechanisms that govern intratumoral T-cell positioning remain poorly defined. Through RNA sequencing of endothelial cells isolated from tumor cores versus peripheries in a mouse melanoma model, we identified intercellular adhesion molecule 1 (ICAM-1) as a candidate regulator of T-cell localization. As tumors progressed, T cells shifted from a balanced core–margin distribution to pronounced core exclusion, coinciding with elevated lymphocyte function-associated antigen-1 (LFA-1) expression on T cells —a pattern less evident in other immune subsets. This redistribution was accompanied by peripheral enrichment of endothelial ICAM-1 and loss of vascular integrity. Functionally, ICAM-1 blockade restored intratumoral T-cell infiltration, enhanced effector activity, and significantly delayed tumor growth. Together, these findings identify endothelial ICAM-1 as a vascular determinant of T-cell positioning and highlight the ICAM-1/LFA-1 axis as a modifiable checkpoint to improve T-cell access to the tumor core and augment antitumor immunity.","dates":{"publication":"2026/09/18"},"accession":"GSE319706","cross_references":{"GSM":["GSM9524136","GSM9524135","GSM9524137","GSM9524132","GSM9524131","GSM9524134","GSM9524133","GSM9524130","GSM9524129"],"GPL":["33896"],"GSE":["319706"],"taxon":["Mus musculus"]}}