{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319903/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319903"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Deciphering the role of oligodendroglial Serpina3n in CPZ pathology using single-nucleus RNA sequencing","description":"Demyelination is a central pathological feature of multiple sclerosis and other neurodegenerative diseases, and the cuprizone (CPZ) diet is a widely used model for studying this process. In this study, we employed single-nucleus RNA sequencing (snRNA-seq) to investigate cellular and transcriptional changes in the corpus callosum during demyelination induced by a 4-week CPZ diet. Samples were collected from three groups: (1) control mice maintained on a normal diet (Norm), (2) demyelinated mice fed a cuprizone diet (CPZ_Ctrl), and (3) cuprizone-treated mice with oligodendrocyte-specific deletion of Serpina3n (Olig2-Cre; Serpina3n flox/flox, CPZ_cKO). This approach enabled dissection of cellular diversity and molecular pathways altered by CPZ-induced demyelination, with a particular focus on the role of oligodendroglial Serpina3n in regulating oligodendrocyte function. Our findings provide insight into cell-type-specific contributions to demyelination and identify Serpina3n as a potential therapeutic target. Overall, this study offers a comprehensive transcriptomic view of oligodendrocyte responses to demyelination, enhancing our understanding of their individual and collective roles in disease pathology.","dates":{"publication":"2026/09/10"},"accession":"GSE319903","cross_references":{"GSM":["GSM9528999","GSM9528998","GSM9528997","GSM9528996","GSM9528995"],"GPL":["28457","34290"],"GSE":["319903"],"taxon":["Mus musculus"]}}