{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319983/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Methylation profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319983"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Targeted RNA N6-Methyladenosine Demethylation inhibits cell migration in colorectal cancer cells [MeRIP-seq]","description":"Colorectal cancer liver metastasis (CRLM) remains a significant challenge in the treatment of colorectal cancer (CRC). N6-methyladenosine (m6A) as a new layer of epigenetic RNA modification is closely linked to various mechanisms of tumor metastasis, offering new avenues for targeted therapies. However, the role of m6A-mediated mechanisms underlying colorectal liver metastasis is still unclear. In this study, we identified a novel m6A-modified transcriptional factor BTB/POZ domain protein ZBTB7A (zinc finger and BTB domain-containing 7A) using integrative m6A-sequencing (MeRIP-seq), RNA sequencing (RNA-seq), and single-cell RNA sequencing (scRNA-seq) analyses, and investigated the functional significance and underlying mechanisms of ZBTB7A as a promoting factor in liver metastasis of CRC. Mechanically, ZBTB7A could be positively regulated by m6A methyltransferase METTL3 and promote the process of CRLM through ARHGAP26-mediated Rho GTPases signaling pathway in a m6A-dependent manner. More importantly, we also applied a targeted RNA m6A erasure (TRME) system to focus methylation on a specific site within a transcript of interest rather altering global levels of m6A abundance and demonstrated that temporal m6A erasure on a single site of ZBTB7A is sufficient to inhibit the CRC cell migration. This study unveils the critical role of the METTL3/ZBTB7A/ARHGAP26 axis in the process of m6A-mediated CRLM and positions m6A precise editing as a promising therapy in the preclinical treatment of tumor metastasis.","dates":{"publication":"2026/09/02"},"accession":"GSE319983","cross_references":{"GSM":["GSM9530804","GSM9530805","GSM9530802","GSM9530803","GSM9530806","GSM9530807","GSM9093379","GSM9093378","GSM9093377","GSM9093376","GSM9093375","GSM9093374","GSM9093373","GSM9093383","GSM9093372","GSM9093382","GSM9093381","GSM9093380"],"GPL":["24676"],"GSE":["319983"],"taxon":["Homo sapiens"],"PMID":["[42649501]"]}}