GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319983/primaryOK200TranscriptomicsHomo sapiens Methylation profiling by high throughput sequencingExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319983GEOGSEfalseTargeted RNA N6-Methyladenosine Demethylation inhibits cell migration in colorectal cancer cells [MeRIP-seq]Colorectal cancer liver metastasis (CRLM) remains a significant challenge in the treatment of colorectal cancer (CRC). N6-methyladenosine (m6A) as a new layer of epigenetic RNA modification is closely linked to various mechanisms of tumor metastasis, offering new avenues for targeted therapies. However, the role of m6A-mediated mechanisms underlying colorectal liver metastasis is still unclear. In this study, we identified a novel m6A-modified transcriptional factor BTB/POZ domain protein ZBTB7A (zinc finger and BTB domain-containing 7A) using integrative m6A-sequencing (MeRIP-seq), RNA sequencing (RNA-seq), and single-cell RNA sequencing (scRNA-seq) analyses, and investigated the functional significance and underlying mechanisms of ZBTB7A as a promoting factor in liver metastasis of CRC. Mechanically, ZBTB7A could be positively regulated by m6A methyltransferase METTL3 and promote the process of CRLM through ARHGAP26-mediated Rho GTPases signaling pathway in a m6A-dependent manner. More importantly, we also applied a targeted RNA m6A erasure (TRME) system to focus methylation on a specific site within a transcript of interest rather altering global levels of m6A abundance and demonstrated that temporal m6A erasure on a single site of ZBTB7A is sufficient to inhibit the CRC cell migration. This study unveils the critical role of the METTL3/ZBTB7A/ARHGAP26 axis in the process of m6A-mediated CRLM and positions m6A precise editing as a promising therapy in the preclinical treatment of tumor metastasis.2026/09/02GSE319983GSM9530804GSM9530805GSM9530802GSM9530803GSM9530806GSM9530807GSM9093379GSM9093378GSM9093377GSM9093376GSM9093375GSM9093374GSM9093373GSM9093383GSM9093372GSM9093382GSM9093381GSM909338024676319983Homo sapiens[42649501]