{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320080/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320080"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell RNA sequencing of hematopoietic and non-hematopoietic cells defines a distinct signature in atopic and NRG1 mouse lung","description":"Respiratory viral infections cause significant mortality. Pre-existing atopy has demonstrated protection from virus-induced death. Mice made atopic with house dust mite (HDM) extract survive normally lethal parainfluenza (Sendai virus, SeV) and influenza A (IAV) viral infection. Neuregulin-1 (NRG1) is markedly elevated in the airways of atopic mice and has been shown to significantly attenuate mortality to SeV and IAV in naïve (non-atopic) mice. Hypothesizing that atopy and NRG1 mediate protection from death through similar mechanisms, we utilized single-cell RNA sequencing data from CD45+ (hematopoietic) and CD45- (structural) lung cell populations to compare atopic versus NRG1-treated mice in an unbiased manner to determine the enrichment of cellular populations and pathways prior to viral infection. Both treatments reduced alveolar macrophages, lymphatic endothelial cells, airway smooth muscle cells, and pericytes, while increasing alveolar type 2 cells. However, atopy led to increased fibroblasts and megakaryocytes with reduced club cells compared to NRG1 treatment. Further, in the hematopoietic cell compartment, atopy drove an increase in more inflammatory cells than NRG1 and associated with significant upregulation of immune cell signaling pathways, something not seen with NRG1 administration. Moreover, NRG1 knockdown did not reverse the protection from virus-induced death in mice with pre-existing atopy. Taken together, these data strongly suggest that atopy and NRG1 mediate increased survival from a respiratory viral infection through disparate mechanisms.","dates":{"publication":"2026/06/30"},"accession":"GSE320080","cross_references":{"GSM":["GSM9533538"],"GPL":["24247"],"GSE":["320080"],"taxon":["Mus musculus"]}}