{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320153/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320153"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A chemical-genetic interaction map of the DNA damage response","description":"The DNA damage response (DDR) is a sophisticated network of cellular pathways whose perturbation leads to genome instability and is a key hallmark of oncogenesis. Here we present data from 32 genome-scale loss-of-function CRISPRi chemical-genetic screens with inhibitors targeting core constituents of the DDR machinery (PARP, ATR, ATM, DNAPK, and WEE1), as both single agents and in combination with PARP inhibitors. These experiments identify >1000 genes whose perturbation modifies the DDR and provides a rich resource to the DDR community. In addition, this compendium of functional genomics data reveals key principles governing the DDR and highlights a strong chemical-genetic interaction between loss of activity of the peroxiredoxin PRDX1 and all tested DDR inhibitors through a mechanism involving iron availability mediated by an MRGBP-PAX7-IREB2 axis. Our data position PRDX1 as a key suppressor of DNA damage accumulation and potential druggable target in combination with DDR inhibitors.","dates":{"publication":"2026/08/24"},"accession":"GSE320153","cross_references":{"GSM":["GSM9535762","GSM9535784","GSM9535785","GSM9535763","GSM9535764","GSM9535786","GSM9535765","GSM9535787","GSM9535780","GSM9535781","GSM9535782","GSM9535760","GSM9535761","GSM9535783","GSM9535788","GSM9535766","GSM9535767","GSM9535768","GSM9535769","GSM9535773","GSM9535751","GSM9535752","GSM9535774","GSM9535753","GSM9535775","GSM9535776","GSM9535754","GSM9535770","GSM9535771","GSM9535772","GSM9535759","GSM9535755","GSM9535777","GSM9535756","GSM9535778","GSM9535779","GSM9535757","GSM9535758"],"GPL":["20301","24676"],"GSE":["320153"],"taxon":["Homo sapiens"]}}