{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320249/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320249"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA sequencing study of iGCB day3 cells to investigate differences between Eif3e cg1cre knockout B cells and control B cells","description":"To obtain a larger number of Eif3e-deficient B cells induced by cg1cre, we utilized an in vitro germinal center B cell (iGCB) culture system. By day 3 of culture, we harvested abundant YFP+ Eif32-deficient B cells and their corresponding control cells. RNA-seq comparison revealed that Eif3e-deficient B cells upregulated some co-stimulatory molecules, including Cd80 and Icam1. GO enrichment analysis showed that upregulated genes were primarily associated with ribosome-related pathways, while downregulated genes were mainly enriched in lipid metabolism. The upregulation of co-stimulatory molecules may confer Eif3e-deficient B cells with the capacity to promote CD4+ T cell activation.","dates":{"publication":"2026/05/01"},"accession":"GSE320249","cross_references":{"GSM":["GSM9538347","GSM9538344","GSM9538343","GSM9538346","GSM9538345","GSM9538342"],"GPL":["24247"],"GSE":["320249"],"taxon":["Mus musculus"]}}