{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320256"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-molecule DNA replication dynamics under WEE1 inhibition in wild-type and TRESLIN-stabilized HCT116 cells","description":"We profiled single-molecule DNA replication dynamics in human HCT116 cells to determine how WEE1 inhibition and stabilization of the origin-firing factor TRESLIN (TICRR) influence replication initiation, termination, and fork progression. We analyzed two engineered HCT116 lines expressing mClover-tagged TRESLIN from the endogenous locus: a wild-type TRESLIN allele and a stabilized ΔSBI TRESLIN mutant. Cells were treated with the WEE1 inhibitor MK-1775 (1 µM) or vehicle and sequentially pulse-labeled with EdU and BrdU, followed by a thymidine chase, to generate replication tracts for long-read nanopore sequencing. High–molecular weight genomic DNA was prepared and sequenced on an Oxford Nanopore PromethION platform, and reads were aligned to the human reference genome (hg38/GRCh38). Replication features (leftward/rightward forks, initiation events, and termination events) and tract lengths were inferred from EdU/BrdU segmentation using DNAscent forkSense, enabling quantification of fork directionality and fork rates across conditions. Associated preprint: bioRxiv [Preprint]. 2025 Jun 11;2025.06.10.657920. doi:10.1101/2025.06.10.657920.","dates":{"publication":"2026/04/17"},"accession":"GSE320256","cross_references":{"GSM":["GSM9538414","GSM9538413","GSM9538405","GSM9538416","GSM9538415","GSM9538410","GSM9538412","GSM9538411","GSM9538407","GSM9538406","GSM9538409","GSM9538408"],"GPL":["26167"],"GSE":["320256"],"taxon":["Homo sapiens"],"DOI":["10.1101/2025.06.10.657920"]}}