GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320324/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320324GEOGSEfalseRSPO2 synergizes with BMP2 to enhance wnt-dependent osteoblast differentiationCanonical Wnt signaling plays a critical role in osteoblast differentiation and bone formation and is enhanced by R-spondin (RSPO) proteins. Bone morphogenetic protein-2 (BMP2) is a clinically used osteogenic factor whose regenerative efficacy is limited by dose-dependent adverse effects. We recently identified RSPO2 as a contextual enhancer of BMP2-induced bone formation, but the transcriptional mechanisms underlying this synergy remain incompletely understood. To define the molecular basis of BMP2–RSPO2 cooperation, we performed RNA sequencing (RNA-seq) analysis of MC3T3-E1 osteoblast precursor cells treated with vehicle control, RSPO2, BMP2, or BMP2+RSPO2 for 5 days under osteogenic conditions. Transcriptomic analysis revealed distinct and overlapping gene expression programs regulated by BMP2 and RSPO2, with co-treatment inducing a unique transcriptional signature enriched for genes associated with osteoblast differentiation, Wnt signaling, and cytoskeletal regulation. These data provide mechanistic insight into how RSPO2 potentiates BMP2-induced osteogenesis and support a role for RSPO2 as a therapeutic co-factor to enhance BMP2-mediated bone regeneration.2026/07/17GSE320324GSM9540702GSM9540703GSM9540704GSM9540705GSM9540706GSM9540707GSM9540708GSM9540709GSM9540699GSM9540710GSM9540700GSM954070124247320324Mus musculus