{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320366/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320366"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Short-Duration HIPEC-Mimetic Exposure to Mithramycin A Induces Structured Transcriptional Reprogramming in HT-29 Colorectal Cancer Cells","description":"This study investigates the transcriptional consequences of short-duration HIPEC-mimetic exposure to Mithramycin A (MA) in HT-29 colorectal cancer cells. Cells were exposed for 90 minutes to MA (750 nM) or Mitomycin C (4.48 µM) at 37 °C followed by drug washout. RNA sequencing was performed to determine whether transient exposure induces durable transcriptional reprogramming. Differential expression and pathway analyses identified coordinated enrichment of cell-cycle checkpoint programs and modulation of chromatin regulatory networks. One biological replicate (MA2) was excluded due to high duplication rates and low mapping efficiency. Analyses excluding this sample are presented.","dates":{"publication":"2026/05/15"},"accession":"GSE320366","cross_references":{"GSM":["GSM9541714","GSM9541715","GSM9541716","GSM9541717","GSM9541718","GSM9541719","GSM9541720","GSM9541721"],"GPL":["24676"],"GSE":["320366"],"taxon":["Homo sapiens"],"PMID":["[42074218]"]}}