GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320366/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320366GEOGSEfalseShort-Duration HIPEC-Mimetic Exposure to Mithramycin A Induces Structured Transcriptional Reprogramming in HT-29 Colorectal Cancer CellsThis study investigates the transcriptional consequences of short-duration HIPEC-mimetic exposure to Mithramycin A (MA) in HT-29 colorectal cancer cells. Cells were exposed for 90 minutes to MA (750 nM) or Mitomycin C (4.48 µM) at 37 °C followed by drug washout. RNA sequencing was performed to determine whether transient exposure induces durable transcriptional reprogramming. Differential expression and pathway analyses identified coordinated enrichment of cell-cycle checkpoint programs and modulation of chromatin regulatory networks. One biological replicate (MA2) was excluded due to high duplication rates and low mapping efficiency. Analyses excluding this sample are presented.2026/05/15GSE320366GSM9541714GSM9541715GSM9541716GSM9541717GSM9541718GSM9541719GSM9541720GSM954172124676320366Homo sapiens[42074218]