GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320482/primary200OKTranscriptomicsSolanum lycopersicumExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320482GEOGSEfalseFar-red perception by vegetative organs and not fruits drives fruit growth responses in tomato plantsResponses to far-red are mediated by phytochromes, specialised photoreceptors present in all organs of a tomato plant. Although fruit-localised phytochromes can influence starch and sugar metabolism, their involvement in modulating fruit growth responses to far-red is unknown. We explored whether fruit growth responses to far-red are driven by local far-red perception within the fruits, or by a systemic response initiated in the vegetative organs. We applied far-red exclusively to the fruiting trusses, from anthesis to ripening, to the vegetative organs, to both, or to neither. We quantified plant development and fruit growth responses to far-red and carried out a transcriptomic analysis to establish the temporal dynamics of far-red perception and signalling in leaves and fruits. Supplementing far-red to the vegetative organs led to a significant increase in ripe fruit weight and sugar concentration, regardless of the light conditions experienced by the trusses, whereas far-red supplementation to the generative organs alone had no measurable effect. This study establishes that far-red perception by vegetative organs drives fruit growth responses in tomato. Local responses to far-red included a transient increase in auxin concentration and upregulation of the auxin-signalling pathway in leaves, a conserved response to decreasing R:FR ratios. In fruits, early transcriptomic responses carried signatures of auxin, cytokinin, and gibberellin biosynthesis and signalling, suggesting a role for hormones as mediators of far-red-induced fruit responses. Finally, this work reveals how responses to far-red perception by vegetative organs promote sink strength and starch biosynthesis in early developing fruits, contributing to higher fruit weight and sugar concentration in ripe fruits.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 lycopersicum[42289860]