GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE321nnn/GSE321693/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE321693GEOGSEfalseDiagnostic performance comparison of SE-SPTM-PCR and miRNA deep sequencing using 2-fold gradient dilutions of simulated HCMV viral infection samplesMicroRNAs are promising liquid biopsy biomarkers, but their clinical translation is hindered by detection challenges, including low abundance, high sequence similarity, and background interference. We developed SE-SPTM-PCR, a detection platform integrating selective miRNA enrichment using locked nucleic acid probes with specific probe terminal-mediated RT-qPCR. To evaluate the ability of the SE-SPTM-PCR platform to discriminate samples with only slight differences in load, we tested simulated specimens with two-fold serial dilutions using both SE-SPTM-PCR and miRNA sequencing. These simulated specimens were prepared by spiking HCMV viral culture supernatant (propagated in MRC5 cells) into plasma samples obtained from three groups of age- and sex-matched healthy donors (n=10 per group). The results demonstrated that the diagnostic performance of the SE-SPTM-PCR platform significantly outperformed that of miRNA sequencing.2026/03/04GSE321693GSM9549666GSM9549688GSM9549667GSM9549689GSM9549668GSM9549669GSM9549684GSM9549685GSM9549663GSM9549664GSM9549686GSM9549687GSM9549665GSM9549680GSM9549681GSM9549682GSM9549683GSM9549677GSM9549678GSM9549679GSM9549673GSM9549674GSM9549675GSM9549676GSM9549691GSM9549692GSM9549670GSM9549671GSM9549672GSM954969024676321693Homo sapiens