GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322507/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322507GEOGSEfalseMELK inhibition promotes megakaryopoiesis and platelet recovery in immune thrombocytopenia through ERK–cofilin signalingMaternal embryonic leucine zipper kinase (MELK) has been implicated in cell-cycle regulation, yet its transcriptional programs in megakaryocytic cells remain incompletely defined. To profile MELK-dependent gene expression changes, we performed bulk RNA-seq on the human megakaryoblastic cell line MEG-01 transduced with a control pLKO.1 vector or a MELK-targeting shRNA (sh2-MELK), with three independent biological replicates per condition. Paired-end sequencing reads (raw FASTQ files) and processed gene-level expression quantifications are provided to support differential expression and pathway analyses of MELK knockdown in megakaryocytic cells.2026/04/02GSE322507GSM9553468GSM9553467GSM9553466GSM9553465GSM9553464GSM955346321290322507Homo sapiens