{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322564/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322564"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Subepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage [RNA-Seq]","description":"The intestinal epithelium relies on the surrounding niche environment to maintain homeostasis, but how the niche supports intestinal stem cell regeneration is unclear. We analyzed a number of distinct mesenchymal populations in the intestinal stem cell niche following two models of murine intestinal injury, including whole body irradiation and diphtheria-toxin injected Lgr5Dtr mice. We isolated Pdgfra fibroblasts from the murine transgenic PDGFRAH2BEGFP (GFP-hi and GFP-lo) and PdgfraCreERT2;Rosa26tDtomato (tDtomato+) mice, smooth muscle from Myh11CreERT2;PdgfraH2BeGFP;Rosa26tDtomato (GFP-tDtomato+), and blood and lympathic endothelium via antibody for CD31+LYVE1- and CD31+LYVE1+, respectively. We found distinct molecular signatures of these cell types following stem cell loss and focused our analyses on known trophic factors of the overlaying intestinal epithelium.","dates":{"publication":"2026/09/18"},"accession":"GSE322564","cross_references":{"GSM":["GSM9554990","GSM9554991","GSM9554992","GSM9554993","GSM9554994","GSM9554995","GSM9554996","GSM9554997","GSM9554998","GSM9554999","GSM9554988","GSM9554989"],"GPL":["19057"],"GSE":["322564"],"taxon":["Mus musculus"]}}