{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322565/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322565"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Subepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage [scRNA-Seq]","description":"The intestinal epithelium relies on the surrounding niche environment to maintain homeostasis, but how the niche supports intestinal stem cell regeneration is unclear. We analyzed a number of distinct mesenchymal populations in the intestinal stem cell niche following two models of murine intestinal injury, including whole body irradiation and diphtheria-toxin injected Lgr5Dtr mice. We isolated Pdgfra fibroblasts from the murine transgenic PDGFRAH2BEGFP (GFP-hi and GFP-lo) and PdgfraCreERT2;Rosa26tDtomato (tDtomato+) mice, smooth muscle from Myh11CreERT2;PdgfraH2BeGFP;Rosa26tDtomato (GFP-tDtomato+), and blood and lympathic endothelium via antibody for CD31+LYVE1- and CD31+LYVE1+, respectively. We found distinct molecular signatures of these cell types following stem cell loss and focused our analyses on known trophic factors of the overlaying intestinal epithelium.","dates":{"publication":"2026/09/18"},"accession":"GSE322565","cross_references":{"GSM":["GSM9555000","GSM9555001","GSM9555002","GSM9555003"],"GPL":["21103"],"GSE":["322565"],"taxon":["Mus musculus"]}}