GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322565/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322565GEOGSEfalseSubepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage [scRNA-Seq]The intestinal epithelium relies on the surrounding niche environment to maintain homeostasis, but how the niche supports intestinal stem cell regeneration is unclear. We analyzed a number of distinct mesenchymal populations in the intestinal stem cell niche following two models of murine intestinal injury, including whole body irradiation and diphtheria-toxin injected Lgr5Dtr mice. We isolated Pdgfra fibroblasts from the murine transgenic PDGFRAH2BEGFP (GFP-hi and GFP-lo) and PdgfraCreERT2;Rosa26tDtomato (tDtomato+) mice, smooth muscle from Myh11CreERT2;PdgfraH2BeGFP;Rosa26tDtomato (GFP-tDtomato+), and blood and lympathic endothelium via antibody for CD31+LYVE1- and CD31+LYVE1+, respectively. We found distinct molecular signatures of these cell types following stem cell loss and focused our analyses on known trophic factors of the overlaying intestinal epithelium.2026/09/18GSE322565GSM9555000GSM9555001GSM9555002GSM955500321103322565Mus musculus