GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322593/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322593GEOGSEfalseContext-dependent effects of DHRS2 on cell-cycle progression, apoptosis and inflammatory gene expression in breast cell linesBasal DHRS2 expression showed significant variation across different cell lines, with the highest levels observed in the non-tumorigenic MCF-10A. Silencing DHRS2 disrupted pathways such as ribosome biogenesis, DNA replication, and ion transport, whereas overexpression altered mitochondrial gene transcription and nucleoside synthesis. Loss of DHRS2 accelerated cell-cycle progression in MCF-7 and MCF-10A cells, whereas its overexpression induced G0/G1 phase arrest. Apoptosis levels increased with knockdown and decreased with overexpression, mainly in cancer cells. Inflammatory gene responses varied depending on cell type, particularly involving CASP1, IL-1A/B, and PYHIN1. Overexpression of DHRS2 promoted cell detachment and migration, suggesting cytoskeletal remodeling.2026/09/22GSE322593GSM9555283GSM9555284GSM9555285GSM9555286GSM9555287GSM9555288GSM9555289GSM9555278GSM9555279GSM9555280GSM9555281GSM955528224676322593Homo sapiens