{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322696"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"snRNA-Seq from abdominal and gluteofemoral human adipose tissue","description":"Adipose tissue biopsies were collected from subcutaneous abdominal and gluteofemadipose tissue from 5 healthy and 5 PCOS women. After isolation, nuclei were counted with a Countess II automated cell counter (Thermofisher Scientific). Single nuclei suspension (56K/mL) was distributed into eight wells of a 384-well source plate (Takara Bio USA, San Jose, CA) and dispensed onto a iCELL8 350v Chip (Takara Bio USA) using an iCELL8 MultiSample NanoDispenser (Takara Bio USA). We distributed the ABD and GF-SVF of each subject on the same chip. In brief, mRNA from single cells were isolated, converted to full-length cDNA prior to unbiased amplification of 3’ and 5’ ends using SMART-Seq® ICELL8® Application Kit (Takara Bio, USA). Multiplexed sequencing libraries were generated from cDNA using the Illumina Nextera XT protocol and 150bp paired-end sequencing was performed on an Novogene NovaSeq X Plus instrument.","dates":{"publication":"2026/06/01"},"accession":"GSE322696","cross_references":{"GSM":["GSM9557033","GSM9557032","GSM9557029","GSM9557031","GSM9557030"],"GPL":["34284"],"GSE":["322696"],"taxon":["Homo sapiens"]}}