GEOTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322696GEOGSEfalsesnRNA-Seq from abdominal and gluteofemoral human adipose tissueAdipose tissue biopsies were collected from subcutaneous abdominal and gluteofemadipose tissue from 5 healthy and 5 PCOS women. After isolation, nuclei were counted with a Countess II automated cell counter (Thermofisher Scientific). Single nuclei suspension (56K/mL) was distributed into eight wells of a 384-well source plate (Takara Bio USA, San Jose, CA) and dispensed onto a iCELL8 350v Chip (Takara Bio USA) using an iCELL8 MultiSample NanoDispenser (Takara Bio USA). We distributed the ABD and GF-SVF of each subject on the same chip. In brief, mRNA from single cells were isolated, converted to full-length cDNA prior to unbiased amplification of 3’ and 5’ ends using SMART-Seq® ICELL8® Application Kit (Takara Bio, USA). Multiplexed sequencing libraries were generated from cDNA using the Illumina Nextera XT protocol and 150bp paired-end sequencing was performed on an Novogene NovaSeq X Plus instrument.2026/06/01GSE322696GSM9557033GSM9557032GSM9557029GSM9557031GSM955703034284322696Homo sapiens