GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322756/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322756GEOGSEfalseEffects of nickel ion treatment on gene expression during osteogenic differentiation of mouse bone marrow mesenchymal stem cellsNickel (Ni) is a ubiquitous environmental trace metal, yet its physiological roles and dose-dependent effects in skeletal biology remain poorly understood. Through comprehensive in vivo and in vitro experiments, we demonstrated that low-dose Ni (0.1 μM) significantly enhances matrix mineralization during osteogenic differentiation, whereas this pro-mineralization effect is markedly attenuated at higher concentrations. Although the inhibitory mechanism of high-dose nickel on osteogenesis has been largely attributed to oxidative stress, the molecular basis underlying the promotional effect of low-dose nickel remains unclear. To address this, we performed RNA-seq analysis on primary mouse bone marrow mesenchymal stem cells (mBMSCs) treated with 0.1 μM nickel following 7 days of osteogenic induction, aiming to elucidate the downstream signaling pathways responsible for its beneficial effects on bone mineralization.2026/06/03GSE322756GSM9557808GSM9557807GSM9557809GSM9557804GSM9557806GSM955780524247322756Mus musculus[42196516]