{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322787/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322787"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A skin-hypothalamus axis couples heat stress and metabolic dysfunction","description":"With the ongoing rise in global temperatures, heat stress is increasingly implicated in chronic metabolic disorders; however, whether a transient high-temperature experience leads to enduring metabolic vulnerability and persistent hypothalamic adaptations remains unclear. To address this question, we established a controlled heat stress (HS) mouse model. Eight-week-old male mice with comparable body weights were assigned to HS or paired-fed (PF) conditions. HS mice were exposed to 37 °C for 6 h during the dark phase for 1 week with ad libitum access to food. PF mice were maintained at room temperature and pair-fed to match the food intake consumed by HS mice during the 6-h exposure window, followed by ad libitum feeding for the remaining 18 h to ensure comparable total daily intake. After HS, mice were returned to room temperature for a 2-week quiescent stage (QS), during which blood glucose and serum corticosterone normalized, indicating recovery from acute stress. Hypothalamic cells were collected from PF, HS, and QS groups for single-cell RNA sequencing to characterize heat stress–associated changes in hypothalamic cellular composition and transcriptional states. We observed a sustained increase in astrocyte abundance after HS that persisted into QS, and identified an expanded astrocyte subpopulation marked by high Lrrc7 expression, providing a foundation for further investigation of persistent hypothalamic responses to heat exposure in the context of metabolic regulation.","dates":{"publication":"2026/04/20"},"accession":"GSE322787","cross_references":{"GSM":["GSM9558484","GSM9558485","GSM9558483"],"GPL":["24247"],"GSE":["322787"],"taxon":["Mus musculus"]}}