{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE323nnn/GSE323983/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE323983"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Senescence inhibition by rapamycin mitigates radiation-induced atherosclerotic characteristics in human coronary endothelial cells","description":"Ionising radiation (IR) is a recognised risk factor for cardiovascular disease (CVD), yet the mechanisms linking it to exposure remain incompletely understood. We show that IR drives a pro-atherogenic phenotype in human coronary artery endothelial cells (HCAECs) through the induction of cellular senescence, and that rapamycin attenuates these effects. IR triggered hallmark senescence features, including elevated SA-β-galactosidase activity, nuclear enlargement, and increased CDKN1A and p53 expression. Functionally, irradiated HCAECs displayed impaired barrier integrity and heightened monocyte adhesion. Transcriptomic and proteomic profiling revealed broad IR-induced alterations enriched in DNA damage response, cell-cycle arrest, senescence, proteostasis, and immune-related pathways. These findings establish a mechanistic link between radiation-induced endothelial senescence and early atherogenic-associated dysfunction, demonstrating that senescence is a driver of vascular injury. Importantly, identifying mTOR inhibition as a promising strategy to counteract radiation-associated endothelial dysfunction. This work positions senescence as a tractable therapeutic target in radiation-induced vascular injury.","dates":{"publication":"2026/06/24"},"accession":"GSE323983","cross_references":{"GSM":["GSM9566095","GSM9566084","GSM9566072","GSM9566093","GSM9566082","GSM9566091","GSM9566080","GSM9566108","GSM9566107","GSM9566105","GSM9566114","GSM9566103","GSM9566112","GSM9566101","GSM9566078","GSM9566089","GSM9566110","GSM9566099","GSM9566087","GSM9566076","GSM9566097","GSM9566086","GSM9566074"],"GPL":["28975"],"GSE":["323983"],"taxon":["Homo sapiens"]}}