{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324100/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324100"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Exercise Training Prior To and During Cancer in Mice Preserves Muscle Mass, Reduces Tumor Weight, and Suppresses Molecular Mediators of Cachexia","description":"We investigated the prophylactic effects of exercise before and during cancer cachexia (CC) using a model designed to mimic endurance and resistance (i.e., concurrent) adaptations. Male and female Balb/c mice were randomly assigned to exercise or control groups whereby exercise groups were subjected to an 8-week voluntary progressive weighted wheel running (PoWeR) program of habitual loading-mediated physical activity beginning at 8 weeks of age. At 16 weeks of age, mice were injected bilaterally with colon-26 adenocarcinoma (C26) cells or PBS, and exercise training was maintained throughout disease progression. Twenty-five days post-tumor induction, we assessed whole-body and muscle phenotype, muscle protein synthesis, a priori targeted gene expression, and transcriptomic adaptations via RNA sequencing. PoWeR training preserved skeletal muscle mass across nearly all muscle groups and maintained tumor-free body and cardiac mass. Muscle mass adaptations related to running volume; and running distance relative to controls was not appreciably reduced by tumor status. Tumor burden was reduced after ~11.5 weeks of PoWeR compared to sedentary, but this was not explanatory for muscle adaptations. PoWeR induced a faster-to-slower muscle fiber type transition in the gastrocnemius and suppressed key protein turnover markers (Redd1, Murf1, Atrogin, Ubc, Gadd45a) as well as the mitophagy-related marker Bnip3 in tumor-bearing muscle; 24h muscle protein synthesis remained stable. PoWeR counteracted tumor-induced impairments in the muscle mitochondrial- and metabolic-related transcriptome. Collectively, physical activity prior to and during cancer preserves muscle mass, reduces tumor growth, and mitigates molecular drivers of CC, underscoring its preventive and therapeutic potential as a lifestyle intervention.","dates":{"publication":"2026/06/10"},"accession":"GSE324100","cross_references":{"GSM":["GSM9568252","GSM9568241","GSM9568253","GSM9568242","GSM9568254","GSM9568243","GSM9568255","GSM9568244","GSM9568256","GSM9568245","GSM9568257","GSM9568246","GSM9568258","GSM9568247","GSM9568259","GSM9568248","GSM9568249","GSM9568260","GSM9568261","GSM9568250","GSM9568251","GSM9568240"],"GPL":["24247"],"GSE":["324100"],"taxon":["Mus musculus"],"PMID":["[42241015]"]}}