GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324334/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324334GEOGSEfalseLong-term Antigen Reduction is Insufficient to Cure Hepatitis B Virus in MiceChronic hepatitis B virus (HBV) infection remains a major cause of mortality, and curative treatments are still lacking. This study investigated whether prolonged suppression of HBV antigens could restore HBV-specific immune responses and support therapeutic vaccination. HBV-carrier mice were treated for up to eight months with liver-directed siRNAs or shRNAs targeting all HBV transcripts. Although viral antigens declined initially, a threshold was reached after four months, and extended suppression alone did not restore T-cell immunity; viral replication rebounded after treatment stopped. However, combining antigen suppression with the therapeutic vaccine TherVacB reconstituted antiviral T-cell responses and achieved sustained HBV control. Longer siRNA pretreatment enhanced interferon signaling and improved vaccine-induced immunity, highlighting the need for combined antiviral suppression and immune stimulation.2026/09/02GSE324334GSM9573822GSM9573821GSM9573831GSM9573820GSM9573830GSM9573826GSM9573825GSM9573824GSM9573823GSM9573819GSM9573829GSM9573818GSM9573817GSM9573828GSM9573827GSM957381634328324334Mus musculus[42379305]