{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324393/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324393"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Polygonum aviculare L. and Its Active Compound Myricetin Inhibit Rheumatoid Arthritis by Targeting Yes","description":"Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and joint destruction. In RA, fibroblast-like synoviocytes (FLS) acquire a pathogenic phenotype in response to inflammatory cytokines, promoting synovial inflammation and tissue destruction. Thus, targeting pathological FLS activation has emerged as a promising therapeutic strategy. Herein, we aimed to identify novel plant-derived candidates capable of suppressing synovial inflammation. Screening of 327 plants identified Polygonum aviculare L. (PA) as a potent inhibitor of IL-17-mediated synovial inflammation. PA attenuated IL-17-, TNF-α-, and IL-1β-mediated pathological responses in RA patient-derived FLS (RA-FLS) and alleviated clinical symptoms and histopathological changes in an animal model. Transcriptomic analysis revealed that PA reversed IL-17-induced gene expression and suppressed inflammation-related pathways, with NF-κB emerging as a central regulatory axis. UHPLC-based compound profiling combined with AI-driven bioactivity prediction identified myricetin as an active compound of PA with anti-inflammatory and NF-κB inhibitory activity. Myricetin exerted anti-RA effects in RA-FLS, and both PA and myricetin attenuated synovial inflammation in a 3D synovium-like micromass culture system. PA and myricetin targeted Yes and inhibited its activity, with subsequent suppression of SHP-2 phosphorylation and downstream signaling pathways. Notably, myricetin directly bound to the ATP-binding site of Yes. These findings demonstrate that PA and myricetin inhibit pathogenic responses through the Yes–SHP-2 signaling axis and highlight their potential as plant-derived therapeutic candidates for RA.","dates":{"publication":"2026/06/10"},"accession":"GSE324393","cross_references":{"GSM":["GSM9574954","GSM9574955","GSM9574960","GSM9574961","GSM9574962","GSM9574956","GSM9574957","GSM9574958","GSM9574959"],"GPL":["24676"],"GSE":["324393"],"taxon":["Homo sapiens"]}}