{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324565/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324565"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing of polysome-associated transcripts in EIF4A1-overexpression and mock-virus APOE4 NGN2 neurons","description":"Polysome-associated RNA was profiled in human NGN2-induced neurons of APOE4 genotype after mock-virus treatment or EIF4A1 overexpression. Neurons were co-plated with rat astrocytes, harvested at 7 weeks post-differentiation, and RNA was isolated from pooled heavy polysome fractions (fractions 17, 18, and 19) following sucrose-gradient fractionation. Libraries were prepared after poly(A) enrichment using SMART-Seq v4 with tagmentation and sequenced as 150-bp paired-end reads on an Illumina NovaSeq X Plus platform.","dates":{"publication":"2026/09/16"},"accession":"GSE324565","cross_references":{"GSM":["GSM9579607","GSM9579605","GSM9579606","GSM9579603","GSM9579604","GSM9579601","GSM9579602","GSM9579599","GSM9579600","GSM9579598"],"GPL":["34284"],"GSE":["324565"],"taxon":["Homo sapiens"]}}