{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324988/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324988"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of BHLHE40 silencing using short-hair pin RNA on CD34+ cells isolated from human cord blood, during TGF-B1 depedent Langerhans cell differentiation","description":"Langerhans cells (LCs) are resident antigen presenting cells of the epidermis whose differentiation, immature state and tissue residency critically depend on transforming growth factor–β1 (TGF-β1) signaling. Although several downstream transcriptional regulators of TGF-β1 have been identified, how LC epithelial identity and homeostasis are transcriptionally stabilized remains incompletely understood.Human CD34⁺ hematopoietic progenitors were differentiated into LCs or monocyte-derived cells in vitro using cytokine-defined conditions, combined with lentiviral knockdown or ectopic expression of BHLHE40. Bulk-RNA seq of shBHLHE40 cells reveals blunted induction of TGF-β1 responsive genes.","dates":{"publication":"2026/05/01"},"accession":"GSE324988","cross_references":{"GSM":["GSM9590039","GSM9590029","GSM9590037","GSM9590038","GSM9590035","GSM9590036","GSM9590033","GSM9590034","GSM9590031","GSM9590032","GSM9590040","GSM9590030"],"GPL":["34284"],"GSE":["324988"],"taxon":["Homo sapiens"]}}