GEOTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324988GEOGSEfalseEffect of BHLHE40 silencing using short-hair pin RNA on CD34+ cells isolated from human cord blood, during TGF-B1 depedent Langerhans cell differentiationLangerhans cells (LCs) are resident antigen presenting cells of the epidermis whose differentiation, immature state and tissue residency critically depend on transforming growth factor–β1 (TGF-β1) signaling. Although several downstream transcriptional regulators of TGF-β1 have been identified, how LC epithelial identity and homeostasis are transcriptionally stabilized remains incompletely understood.Human CD34⁺ hematopoietic progenitors were differentiated into LCs or monocyte-derived cells in vitro using cytokine-defined conditions, combined with lentiviral knockdown or ectopic expression of BHLHE40. Bulk-RNA seq of shBHLHE40 cells reveals blunted induction of TGF-β1 responsive genes.2026/05/01GSE324988GSM9590039GSM9590029GSM9590037GSM9590038GSM9590035GSM9590036GSM9590033GSM9590034GSM9590031GSM9590032GSM9590040GSM959003034284324988Homo sapiens