{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325085/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325085"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of NF-ĸB and JAK inhibition in a 3D spheroid model of rheumatoid arthritis (RA) synovial tissue incorporating RA fibroblast-like synoviocytes, macrophages and endothelial cells","description":"This study uses a human multicellular 3D spheroid model to investigate inflammatory and angiogenic mechanisms in rheumatoid arthritis (RA) synovial tissue and to evaluate responses to targeted kinase inhibitors. Human M1- or M2-polarized macrophages were co-cultured with RA fibroblast-like synoviocytes (RAFLS) and endothelial cells (HUVECs) to generate spheroids embedded in a collagen-based matrix (n=4). Spheroids were exposed to inflammatory stimuli (20%SF) and pharmacological inhibitors including JAK1/3 inhibitor tofacitinib (5μM) and NF-ĸB pathway inhibitors IKKβi (2.5μM) and NIKi (5μM). Bulk-RNA sequencing was performed to characterize transcriptional responses across experimental conditions.","dates":{"publication":"2026/09/01"},"accession":"GSE325085","cross_references":{"GSM":["GSM9595684","GSM9595683","GSM9595686","GSM9595685","GSM9595688","GSM9595687","GSM9595701","GSM9595700","GSM9595689","GSM9595680","GSM9595682","GSM9595681","GSM9595673","GSM9595695","GSM9595694","GSM9595672","GSM9595697","GSM9595675","GSM9595696","GSM9595674","GSM9595699","GSM9595677","GSM9595676","GSM9595698","GSM9595679","GSM9595678","GSM9595691","GSM9595690","GSM9595693","GSM9595692"],"GPL":["24676"],"GSE":["325085"],"taxon":["Homo sapiens"]}}