GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325085/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325085GEOGSEfalseEffect of NF-ĸB and JAK inhibition in a 3D spheroid model of rheumatoid arthritis (RA) synovial tissue incorporating RA fibroblast-like synoviocytes, macrophages and endothelial cellsThis study uses a human multicellular 3D spheroid model to investigate inflammatory and angiogenic mechanisms in rheumatoid arthritis (RA) synovial tissue and to evaluate responses to targeted kinase inhibitors. Human M1- or M2-polarized macrophages were co-cultured with RA fibroblast-like synoviocytes (RAFLS) and endothelial cells (HUVECs) to generate spheroids embedded in a collagen-based matrix (n=4). Spheroids were exposed to inflammatory stimuli (20%SF) and pharmacological inhibitors including JAK1/3 inhibitor tofacitinib (5μM) and NF-ĸB pathway inhibitors IKKβi (2.5μM) and NIKi (5μM). Bulk-RNA sequencing was performed to characterize transcriptional responses across experimental conditions.2026/09/01GSE325085GSM9595684GSM9595683GSM9595686GSM9595685GSM9595688GSM9595687GSM9595701GSM9595700GSM9595689GSM9595680GSM9595682GSM9595681GSM9595673GSM9595695GSM9595694GSM9595672GSM9595697GSM9595675GSM9595696GSM9595674GSM9595699GSM9595677GSM9595676GSM9595698GSM9595679GSM9595678GSM9595691GSM9595690GSM9595693GSM959569224676325085Homo sapiens