{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325137/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325137"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Type II IL-4 receptor complex drives mesenchymal fibroblast activation in keloids via STAT6 signaling","description":"Keloids result from aberrant wound healing characterized by excessive collagen and mesenchymal-related protein production. The type II IL-4 receptor complex, composed of IL-4Rα and IL-13Rα1, is implicated in keloid pathogenesis but remains underexplored. To address this, we applied single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) to healthy normal skin, adjacent normal skin, active keloids, and regressed keloids. Integrated scRNA-seq and ST analyses identified IL4R+/IL13RA1+/POSTN+ fibroblasts with combined reticular and mesenchymal phenotypes that were enriched in fibrotic regions of keloids. Immunofluorescence studies confirmed co-localization of IL4R, IL13RA1, and POSTN proteins in keloid tissue. Elevated mRNA levels of IL4R, IL13RA1, COL1A1, FN1, and POSTN were found in both keloid tissue and fibroblasts compared to normal skin. Furthermore, silencing IL13RA1 or IL4R using siRNA in keloid fibroblasts (KFs) resulted in reduced expression of COL1A1, FN1, and POSTN. Lastly, inhibiting type II receptor signaling with dupilumab, an anti-IL4R monoclonal antibody, suppressed IL-13-induced COL1A1, FN1, and POSTN protein expression in KF and collagen deposition in keloid explants. In conclusion, our in vitro and ex vivo findings suggest that inhibition of type II IL-4 receptor signaling with dupilumab may reduce mesenchymal protein expression and collagen deposition, warranting further investigation of this pathway in keloid disease.","dates":{"publication":"2026/10/01"},"accession":"GSE325137","cross_references":{"GSM":["GSM9596630","GSM9596632","GSM9596631","GSM9596634","GSM9596633","GSM9596636","GSM9596625","GSM9596635","GSM9596624","GSM9596627","GSM9596626","GSM9596629","GSM9596628"],"GPL":["24676"],"GSE":["325137"],"taxon":["Homo sapiens"]}}