GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325386/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325386GEOGSEfalseSocial hierarchy shapes behavioral and transcriptional responses to chronic stress and ketamine in male miceChronic stress is a major risk factor for psychiatric disorders, yet the mechanisms underlying individual variability in stress vulnerability and treatment response remain poorly understood. Here, we combined a novel behavioral phenotyping system with high-throughput gene expression profiling to examine how chronic stress and ketamine treatment interact over time. Bulk RNA sequencing was performed in the medial prefrontal cortex and ventral hippocampus, revealing a hierarchy-dependent transcriptional response with 141 differentially expressed genes in the medial prefrontal cortex of dominant, ketamine-treated mice, enriched in synaptic and postsynaptic compartments. These findings identify synaptic remodeling as a substrate for ketamine’s sustained antidepressant effects and underscore social rank as a critical factor when dissecting molecular responses to stress and treatment.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 musculus[42495548]